MAP3K6 (Human) Recombinant Protein
产品名称： MAP3K6 (Human) Recombinant Protein
英文名称： MAP3K6 (Human) Recombinant Protein
产品编号： P5592
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- Specification
- Product Description:
- Human MAP3K6 (NP_004663.3, 622 a.a. - 950 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
- Theoretical MW (kDa):
- 63
- Form:
- Liquid
- Preparation Method:
- Baculovirus infected insect cell (Sf21) expression system
- Purification:
- Glutathione sepharose chromatography
- Purity:
- 88 % by SDS-PAGE/CBB staining
- Activity:
- The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG. Substrate: MAP2K7 [inactive mutant]. ATP: 100 uM.
- Storage Buffer:
- In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
- Storage Instruction:
- Store at -80°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Loading 1 ug protein in SDS-PAGE
- Note:
- Result of activity analysis
-
- Application Image
- Functional Study
- SDS-PAGE
- Entrez GeneID:
- 9064
- Protein Accession#:
- NP_004663.3
- Gene Name:
- MAP3K6
- Gene Alias:
- ASK2,MAPKKK6,MGC125653,MGC20114
- Gene Description:
- mitogen-activated protein kinase kinase kinase 6
- Omim ID:
- 604468
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a member of the serine/threonine protein kinase family. The encoded kinase was identified by its interaction with MAP3K5/ASK, a protein kinase and an activator of c-Jun kinase (MAPK7/JNK) and MAPK14/p38 kinase. This kinase was found to weakly activate MAPK7, but not MAPK1/ERK or MAPK14. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined. [provided by RefSeq
- Other Designations:
- OTTHUMP00000005063,apoptosis signal-regulating kinase 2
- Gene Pathway