MCM2 monoclonal antibody, clone 1E7
产品名称： MCM2 monoclonal antibody, clone 1E7
英文名称： MCM2 monoclonal antibody, clone 1E7
产品编号： MAB10806
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against partial recombinant MCM2.
- Immunogen:
- Recombinant protein corresponding to human MCM2.
- Host:
- Mouse
- Reactivity:
- Human
- Form:
- Liquid
- Isotype:
- IgG1
- Storage Buffer:
- In ascites (0.03% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis using MCM2 monoclonal antibody, clone 1E7 (Cat # MAB10806) against MCF-7 (1), HeLa (2), Jurkat (3), K-562 (4), HEK293 (5) and HEPG2 (6) cell lysate.
- Western Blot (Recombinant protein)
- Western blot analysis using MCM2 monoclonal antibody, clone 1E7 (Cat # MAB10806) against human MCM2 (aa: 16-232) recombinant protein. (Expected MW is 50.4 kDa)
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Immunohistochemical analysis of paraffin-embedded human ovarian cancer (A) and human colon cancer (B) tissues using MCM2 monoclonal antibody, clone 1E7 (Cat # MAB10806) with DAB staining.
- Immunofluorescence
- Immunofluorescence analysis of HeLa cells using MCM2 monoclonal antibody, clone 1E7 (Cat # MAB10806) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. MCM2 monoclonal antibody, clone 1E7 (Cat # MAB10806) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Entrez GeneID:
- 4171
- Gene Name:
- MCM2
- Gene Alias:
- BM28,CCNL1,CDCL1,D3S3194,KIAA0030,MGC10606,MITOTIN,cdc19
- Gene Description:
- minichromosome maintenance complex component 2
- Omim ID:
- 116945
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. [provided by RefSeq
- Other Designations:
- DNA replication licensing factor MCM2,MCM2 minichromosome maintenance deficient 2, mitotin,cell devision cycle-like 1,cyclin-like 1,minichromosome maintenance deficient 2 (mitotin),nuclear protein BM28
- Gene Pathway
- Related Disease