HDAC2-SPECIFIC抗体
Applications
In-house applications |
ELISA, WB, IHC, IF, IP Application not tested? Please see our Guarantee. |
Species specificity |
Human,Mouse,Rat; other species not tested. |
Images
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- HepG2 cells were subjected to SDS PAGE followed by western blot with 16152-1-AP(HDAC2-specific antibody) at dilution of 1:500
- Immunohistochemical of paraffin-embedded human testis using 16152-1-AP(HDAC2-specific antibody) at dilution of 1:50 (under 10x lens)
- Immunohistochemical of paraffin-embedded human testis using 16152-1-AP(HDAC2-specific antibody) at dilution of 1:50 (under 40x lens)
- Immunofluorescent analysis of HeLa cells using 16152-1-AP( HDAC2-specific Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
- IP Result of anti-HDAC2-specific (IP:16152-1-AP, 4ug; Detection:16152-1-AP 1:500) with A431 cells lysate 2400ug.
|
Positive WB detected in |
HepG2 cells, A431 cells, HeLa cells, human liver tissue |
Positive IP detected in |
A431 cells |
Positive IHC detected in |
Human testis tissue, human brain tissue, human heart tissue, human kidney tissue, human liver tissue, human ovary tissue, human skin tissue, human spleen tissue |
Positive IF detected in |
HeLa cells |
Recommended dilution |
WB : 1:500-1:5000
IP : 1:200-1:2000
IHC : 1:20-1:200
IF : 1:10-1:100 |
HDAC2-SPECIFIC抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source |
Rabbit |
Purification method |
Antigen affinity purification |
Isotype |
IgG |
Storage |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
Immunogen |
Peptide |
Full name |
Histone deacetylase 2 |
Calculated molecular
weight |
55 kDa |
Observed molecular
weight |
55 kDa |
GenBank accession
number |
NM_001527 |
Gene ID (NCBI) |
3066 |
Gene symbol |
HDAC2 |
Synonyms |
HD2, HDAC2, histone deacetylase 2, RPD3, YAF1 |
Background HDAC2-SPECIFIC抗体
Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human HDAC2 and is specific to HDAC2. It will not cross react with other HDACs. HDAC2-SPECIFIC抗体
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