Anti-SAPK/JNK Mouse Monoclonal Antibody-其它资料-资讯-生物在线

Anti-SAPK/JNK Mouse Monoclonal Antibody

作者：上海康成生物工程有限公司 2008-01-30T00:00 (访问量:1739)

Anti-SAPK/JNK Mouse Monoclonal Antibody

Catalog No: KC-5S04

Size: 100 µl (10 Western mini-blots)

Applications	Species Cross-Reactivity	Molecular Wt.	Source
W	H, M, R	46, 54 kDa	Mouse

Background:

Western blot analysis of extracts from HeLa, NIH/3T3, PC12 and COS cells, using SAPK/JNK (56G8) Rabbit Monoclonal Antibody.

The stress-activated protein kinase/Jun-N-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, inflammatory cytokines and, in some instances, by growth factors and GPCR agonists. As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-4, or by one of the mixed lineage kinases (MLKs) that phosphorylate and activate MKK4-7, the SAPK/JNK kinase. Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs. Alternatively, MKK4-7 can be activated by a pathway independent of small GTPases via stimulation of a member of the germinal center kinase (GCK) family. There are three SAPK/JNK genes with further diversification resulting from alternative splicing. SAPK/JNK, when active as a dimer, can translocate to the nucleus where it regulates transcription through its effects on c-Jun, ATF-2 and other transcription factors.

Specificity/Sensitivity:

SAPK/JNK Rabbit Monoclonal Antibody detects endogenous levels of total SAPK/JNK protein.

Source/Purification:

Monoclonal antibody is produced by immunizing mice with an epitope mapping to amino acids 1-424 representing full length JNK2 of human origin.

Storage and Stability:

Each vial contains 100ul affinity purified rabbit anti-SAPK/JNK antibody in a special stabilizer. Real time studies that this special stabilizer helped retain most of antibody activity for 24 months while store at 4℃.

Recommended Antibody Dilutions:

Western blotting

1:1000

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